Product Supply Code: NLAB-CHEMICALSIND21025
Product Name: Acetamide Broth Twin Pack
Tender Supply Category: Dehydrated Culture Media
Acetamide Broth Twin Pack
Acetamide Broth Twin Pack is recommended for confirmation Pseudomonas aeruginosa in water samples.
Directions
Suspend 7.63 grams of part B in 1000 ml distilled water. Add 10.0 grams of Part A. Heat if necessary to dissolve the medium completely. Dispense in 10ml amounts in tubes or as desired. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.
Principle And Interpretation
Acetamide Broth is formulated as per the recommendation of Standard Methods for the Examination of Water and Wastewater. Acetamide is utilized by a wide variety of non-fermenting organisms. This unique ability is useful in identification of various non-fermenting gram-negative organisms. The media contains inorganic salts and acetamide a sole carbon and nitrogen source. However very few organisms growing in the medium metabolize acetamide by the process of deamination. Acetamide deamination leads to the liberation of ammonia, which thereby increases the pH of the medium, leading to a subsequent colour change of the phenol red indicator from yellow orange to purplish red. Sodium chloride maintains osmotic equilibrium. Phenol red is the pH indicator. Phosphates in the media serve as buffering agents, Magnesium sulphate is a source of ions that stimulate metabolism whereas Acetamide serves as the sole nitrogen and carbon source.
Quality Control
Reaction: Reaction of the medium (Mixture of 1% w/v Part A and 0.76% w/v of Part B) at 25°C. pH : 7.0±0.2
Colour and Clarity of prepared medium: Orange coloured clear solution in tubes
pH: 6.80-7.20
Appearance:
Part A : Colourless deliquescent crystals
Part B : Light yellow to light pink homogeneous free flowing powder.
Acetamide Broth Twin Pack Quality Assurance: We being a leading Acetamide Broth Twin Pack manufacturer from India. NaugraLabEquipments are actively involved in providing precision equipments of premium quality to clients across the globe for Testing Lab and TVET Technical Lab, Industial Training Institutions. We always utilize the most advanced and sophisticated technology for production process of Acetamide Broth Twin Pack and supply exceptional range of other products like Acetamide Broth Twin Pack for tender supply from India.
We supply Acetamide Broth Twin Pack for Bidding and Tendering Turnkey Projects. We supply all laboratory products related to Ministry of Education and Ministry of Health, Medical and Hospital Lab Tenders, Material Testing Lab, School Science Scientific Instruments, Lab Teaching and Educational Lab Equipments, TVET Technical and Vocational Education Training Lab. Manufacturers of Acetamide Broth Twin Pack from India.
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